Table 1.

Sequences of oligonucleotides used for amplification of target genes

Gene (protein)a	Primer (sequence)
Cbei_R0001 (16S rRNA)	16S-F (5′-GAAGAATACCAGTGGCGAAGGC-3′)
	16S-R (5′-ATTCATCGTTTACGGCGTGGAC-3′)
Cbei_2110 (ArsA)	ArsA-F (5′-GGGCTTGAAAACGTAAGAGC-3′)
	ArsA-R (5′-CTCCGCCTTTACCCATTGTA-3′)
Cbei_1273 (hypothetical protein)	hp1-F (5′-ATTAGAGCAGGCGTTTTGGA-3′)
	hp2-R (5′-AACCTTCTTTCAGCCGCATA-3′)
Cbei_0476 (hypothetical protein)	hp2-F (5′-GCAAGCAGGCTTCTAGCATT-3′)
	hp2-R (5′-CAGAAATGCAAATTGCTCCA-3′)
Cbei_0422 (SpoIID)	spoIID-F (5′-TGATACAATTACTGCCAAG-3′)
	spoIID-R (5′-GTTTTTCCAGAGCTGGTTGC-3′)
Cbei_2146 (PdaA)	pdaA-F (5′-CATGAGAAGGATTGGCATCA-3′)
	pdaA-R (5′-TGGTAAAGCCTTCGCTGTTT-3′)
Cbei_0830 (DnaK)	dnak-F (5′-ACTAAGGATGCAGGTAAG-3′)
	dnaK-R (5′-CACCACCTAAGTCATAAA-3′)
Cbei_1049 (ESBP)	espb-F (5′-TTGGGAGTAAGCTTGGTTGC-3′)
	esbp-R (5′-TGGAACTATTTCCCCGTTCTC-3′)
Cbei_0829 (GrpE)	grpE-F (5′-GCTGTTGCAGCTGATGGTAG-3′)
	grpE-R (5′-CCCTTTTGGAAAACTTCTGC-3′)
Cbei_2831 (CBP)	cbp-F (5′-GCAGCAACAACAAAAGCAGA-3′)
	cbp-R (5′-GGGTTATATGGTGCTGTTCC-3′)

^aESBP, extracellular solute-binding protein.