(A and B) Verification of the genomic structure of the NeuroBactrus and its recombinants by PCR and Southern blot analyses. (A) The polyhedrin, cry1-5, and AaIT genes were PCR amplified using specific primer sets. (B) Viral DNA samples were digested with SalI and electrophoresed on a 0.8% agarose gel. The DNAs were hybridized with a labeled polyhedrin gene probe. Lanes: M, 100-bp DNA ladder; wt, wild-type AcMNPV; 1, NeuroBactrus; 2, NBt-DelA; 3, NBt-Del5; 4, NBt-DelA5.