Table 3.
qPCR analysis for the transcription of AaIT and cry1-5 in S. exigua larvae infected with serially passaged NeuroBactrusa
| Virus | Passage |
AaIT |
cry1-5 |
||
|---|---|---|---|---|---|
| RTL (10−5) | SD (10−5) | RTL (10−3) | SD (10−3) | ||
| AcMNPV | — | — | — | — | |
| NeuroBactrus | P0 | 3.70 | 0.43 | 2.66 | 1.2 |
| P2 | 0.64 | 0.28 | 0.13 | 0.05 | |
| P4 | 0.10 | 0.04 | — | — | |
a
Relative transcription level (RTL) data from qPCR run in triplicate were normalized to the transcription of the polyhedrin gene. For comparison of the data, the results were adjusted to approximately 45 (AaIT/polh) or 40 (cry1-5/polh) arbitrary units. –, not detected.