Table 3.
Siderophore name (m/z; retention time [min]) | Type |
Trichoderma genes involved in siderophore biosynthesis |
Reference(s) | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Gene name(s) |
T. virens |
T. atroviride |
T. reesei |
Annotation | ||||||
GenBank no. | E valueb | GenBank no. | E value | GenBank no. | E value | |||||
All | All | sidA | EHK26838 | 2E−162 | EHK44669 | 2E−144 | EGR44632 | 1E−161 | The initial biosynthetic step, shared by pathways of both intra- and extracellular siderophore biosynthesis, is catalyzed by the ornithine-N5-monooxygenase SidA (in A. fumigatus XP_755103). | 50 |
Ferricrocin (771.248; 14.6) | Ferichrome | sidL | EHK18514 | 5E−145 | EHK46975 | 2E−144 | EGR46972 | 9E−135 | Biosynthesis of ferricrocin and hydroxyferricrocin involves acetylation of N5-hydroxyornithine to N5-acetyl-N5-hydroxyornithine by SidL in A. fumigatus (XP_750193). | 49 |
sidC, sid2, NPS2 | EHK26839 | 0E+00 | EHK44670 | 0E+00 | EGR44663 | 0E+00 | In A. fumigatus, NRPS SidC (XP_753088; Sid2 in U. maydis) couples 3 acetylhydroxyornithine molecules to 1 serine and 2 glycine residues to yield cyclic ferricrocin. | |||
Coprogen (822.309; 18.2) Fusigen (780.299; 14.3) cis-fusarin (574.193; 10.0) Fusarinine Aa (556.183; 8.5) Fusarinine Ba (798.309; 12.7) Dimerum acid (538.172; 10.7) | Fusarinine/coprogen | sidI | EHK18683 | 0E+00 | EHK45574 | 0E+00 | EGR46098 | 0E+00 | Acyl-CoA ligase SidI in A. fumigatus (XP_753087) converts mevalonate to mevalonyl-CoA. | 51 |
sidH | EHK21207, EHK20720, EHK20161 | 2E−102, 3E−89, 8E−85 | EHK46085 | 3E-87 | EGR44125, EGR44857, EGR49665 | 4E−98, 2E−91, 3E−84 | Enoyl-CoA hydratase SidH in A. fumigatus (XP_748661) converts mevalonyl-CoA to anhydromevalonyl-CoA. | 51 | ||
sidF | EHK21208 | 0E+00 | EHK45575 | 3E−129 | EGR44134 | 0E+00 | In A. fumigatus, anhydromevalonyl-CoA is transferred to N5-hydroxyornithine by the transacylase SidF (XP_748660) that is required for biosynthesis of fusarinine C and triacetylfusarinine C. | 50 | ||
NPS6 | EHK18682 | 0E+00 | EHK46196 | 0E+00 | EGR46022 | 0E+00 | NRPS related to NPS6 siderophore of Cochliobolus heterostrophus (AAX09988); NPS6 might produce a siderophore corresponding to fusarinine of A. nidulans, A. oryzae, and F. graminearum and coprogen in Neurospora crassa based on the domain structure of the predicted protein and sequence homology. | 48c | ||
sidD | ID 70206/EHK21211 | 0E+00 | ID 71005/EGR44132 | 0E+00 | Siderophore synthetase SidD, homologous to NPS6 of Trichoderma sp. NRPS SidD in A. fumigatus (XP_748662), transfers N5-cis-anhydromevalonyl-N5-hydroxy-l-ornithine to fusarinine C. | 50c | ||||
sidG | EHK22271 | 2E−62 | Acetyltransferase SidG is required for conversion of fusigen (fusarinine C) into triacetylfusarinine C in A. fumigatus (XP_748685). | 50, 51 |
Unambiguous annotation not possible.
E values refer to the query accession number listed in annotation.
Also Trichoderma genome annotation (http://genome.jgi-psf.org/pages/home.jsf?query=Trichoderma&searchType=Keyword).