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SSDH activity in E. coli and L. monocytogenes 10403S. Crude cell extracts of either E. coli K-12, JD23574, JD23574::pKAK0913, 10403S, or the 10403S Δlmrg_02013 or 10403S Δlmrg_02013 pKAK0913 mutant were harvested after 16 to 18 h of growth and incubated with 1.0 mM SSA. NADPH production was measured as the OD340. Values are the means of data from two individual culture replicates, each measured twice. Errors bars shown display the deviations of values seen between each replicate sample.
