Table 2.

PCR amplification conditions for V. cholerae, V. parahaemolyticus, the V. parahaemolyticus toxin gene, and V. vulnificus

Primer pair(s)	Conditions
	Denaturing cycle	Repetitive cycle			Elongation cycle
		1	2	3
PVC-F2/PVCM-R1	94°C, 5 min	94°C, 0.5 min	60°C, 1 min	72°C, 0.5 min	72°C, 7 min
tlhF/tlhR and VVF/VVR	94°C, 3 min	94°C, 1 min	65°C, 1 min	72°C, 1 min	72°C, 5 min
tdhF/tdhR	94°C, 3 min	94°C, 1 min	60°C, 1 min	72°C, 1 min	72°C, 5 min