Table 2.
Identified protein entries related to terephthalate degradation, methanogenesis, signal transduction, and motility of Pelotomaculum, Methanosaeta, and Methanolinea in terephthalate-degrading biofilm at 50°C
| Gene ID | Protein annotation | Putative mechanism involvementa | Sequence coverageb (%) | Peptide hitc (no.) | Microorganismd |
|---|---|---|---|---|---|
| tadcc27178 | UbiD family decarboxylases | TA degradation | (5.8, 38.7) | (7, 273) | Pelotomaculum |
| tadcc30311 | Cyclohexa-1,5-dienecarbonyl-CoA hydratase | TA degradation | 46.0 | 69 | Pelotomaculum |
| tadcc30312 | 6-Hydroxycyclohex-1-ene-1-carboxyl-CoA dehydrogenase | TA degradation | 14.7 | 44 | Pelotomaculum |
| tadcc30310 | 6-Oxo-cyclohex-1-ene-carbonyl-CoA hydrolase | TA degradation | (2.1, 27.7) | (6, 187) | Pelotomaculum |
| tadcc30320 | Crotonase | TA degradation | 56.5 | 115 | Pelotomaculum |
| tadcc30319 | β-Hydroxybutyryl-CoA dehydrogenase | TA degradation | 11.0 | 10 | Pelotomaculum |
| tadcc30308 | Acetyl-CoA acetyltransferase | TA degradation | (6.5, 35.7) | (14, 86) | Pelotomaculum |
| tadcc30318 | Acetyl-CoA acetyltransferase | TA degradation | 41.4 | 55 | Pelotomaculum |
| tadcc30313 | Butyryl-CoA dehydrogenase | TA degradation | 38.4 | 102 | Pelotomaculum |
| tadcc12813 | Fe-only hydrogenase | TA degradation | 3.5 | 2 | Pelotomaculum |
| tadcc23108 | Zinc-finger protein HypA/HybF | TA degradation | 8.0 | 2 | Pelotomaculum |
| tadcc16835 | PAS-containing transcriptional regulator | Signaling | 3.38 | 2 | Pelotomaculum |
| tadcc24013 | Diguanylate cyclase (GGDEF) domain | Signaling | (6.1, 6.1) | (1, 2) | Pelotomaculum |
| tadcc35368 | PAS/PAC sensor signal transduction histidine kinase | Signaling | 2.6 | 1 | Pelotomaculum |
| tadcc7371 | Methyl-accepting chemotaxis protein | Signaling/motility | 13.2 | 61 | Pelotomaculum |
| tadcc23087 | Methyl-accepting chemotaxis protein | Signaling/motility | 6.91 | 4 | Pelotomaculum |
| tadcc12422 | Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain | Signaling/motility | 8.7 | 1 | Pelotomaculum |
| tadcc5742 | Universal stress protein UspA and related nucleotide-binding proteins | Signaling | 9.5 | 1 | Pelotomaculum |
| tadcc18253 | Flagellin and related hook-associated proteins | Motility | 12.6 | 1 | Pelotomaculum |
| tadcc27522 | Acetyl-coenzyme A synthetase | HAc methanogenesis | 8.2 | 29 | Methanosaeta |
| tadcc27524 | Acetyl-coenzyme A synthetase | HAc methanogenesis | 4.4 | 12 | Methanosaeta |
| tadcc32283 | Acetyl-coenzyme A synthetase | HAc methanogenesis | 6.4 | 10 | Methanosaeta |
| tadcc17916 | Acetyl-CoA decarbonylase/synthase alpha subunit | HAc methanogenesis | 6.3 | 24 | Methanosaeta |
| tadcc8403 | Acetyl-CoA decarbonylase/synthase beta subunit | HAc methanogenesis | 6.2 | 68 | Methanosaeta |
| tadcc35500 | Formylmethanofuran dehydrogenase, subunit A | Methanogenesis | 3.9 | 1 | Methanosaeta |
| tadcc1199 | Formylmethanofuran dehydrogenase subunit B | Methanogenesis | 10.29 | 1 | Methanosaeta |
| tadcc28098 | Tetrahydromethanopterin S-methyltransferase, subunit A | Methanogenesis | (6.6, 11.5) | (6, 13) | Methanosaeta |
| tadcc28101 | Tetrahydromethanopterin S-methyltransferase, subunit H | Methanogenesis | 5.8 | 6 | Methanosaeta |
| tadcc11668 | Methyl-coenzyme M reductase, alpha subunit | Methanogenesis | (14.2, 17.4) | (5, 51) | Methanosaeta |
| tadcc5364 | Methyl-coenzyme M reductase, alpha subunit | Methanogenesis | (6.6, 15.0) | (17, 54) | Methanosaeta |
| tadcc5367 | Methyl-coenzyme M reductase, beta subunit | Methanogenesis | 23.5 | 36 | Methanosaeta |
| tadcc6056 | Methyl-coenzyme M reductase, beta subunit | Methanogenesis | (26.5, 26.5) | (6, 204) | Methanosaeta |
| tadcc6057 | Methyl-coenzyme M reductase, beta subunit | Methanogenesis | (7.0, 24.4) | (9, 203) | Methanosaeta |
| tadcc6054 | Methyl-coenzyme M reductase, gamma subunit | Methanogenesis | 32.9 | 61 | Methanosaeta |
| tadcc5365 | Methyl-coenzyme M reductase, gamma subunit | Methanogenesis | 11.1 | 628 | Methanosaeta |
| tadcc3596 | Methyl-coenzyme M reductase, beta subunit | Methanogenesis | 5.03 | 99 | Methanolinea |
| tadcc3599 | Methyl-coenzyme M reductase, gamma subunit | Methanogenesis | (7.1, 7.1) | (3, 8) | Methanolinea |
| tadcc3700 | F420-reducing hydrogenase, gamma subunit | Methanogenesis | 13.5 | 18 | Methanolinea |
| tadcc3028 | Universal stress protein UspA and related nucleotide-binding proteins | Signaling | 5.7 | 64 | Methanolinea |
| tadcc3181 | Chemotaxis protein histidine kinase and related kinases | Signaling/motility | 4.0 | 1 | Methanolinea |
| tadcc39810 | Osmosensitive K+ channel histidine kinase | Signaling | 13.8 | 1 | Methanolinea |
a
TA degradation, anaerobic degradation pathway of terephthalate; HAc methanogenesis, acetoclastic methanogenesis pathway.
b
That is, the percentage of the protein sequence covered by identified peptides. The numbers of duplicated results are indicated in parentheses.
c
That is, the number of peptide sequences matched to the protein group. Numbers of duplicated results are indicated in parentheses.
d
The microbial origins detected with the IMG/M metagenomic database of syntrophic terephthalate-degrading biofilm.