Fig 1.

Activity of SPLUNC1 against Pseudomonas aeruginosa-pMF230. (A) P. aeruginosa-pMF230 was grown with or without SPLUNC1. Bacterial CFU with 1 μg or 5 μg of SPLUNC1 or in the presence of buffer, as a negative control, were measured. The growth reduction of P. aeruginosa-pMF230 was reproducible in repeated experiments. (B) P. aeruginosa-pMF230 was grown with or without SPLUNC1 for 24 h. Bacterial CFU with 1 μg of SPLUNC1 or in the presence of buffer, as a negative control, were measured. All experiments were performed at least 3 times, and data are presented as means ± SD. Both graphs showed statistical significance (*, P < 0.005; **, P < 0.01) of the differences between the SPLUNC1 protein and buffer control results. #, P < 0.01 (1 μg versus 5 μg for the SPLUNC1-treated groups).