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. 2013 Jan;81(1):201–208. doi: 10.1128/IAI.00991-12

Fig 2.

Fig 2

Complementation of FTL_0699::Tn5 and FTL_0724::Tn5 mutants with FTL_0699 and FTL_0724, respectively, restored normal induction of IL-1β and IL-18 secretion. (A and B) LPS-primed BMMϕ were challenged at an MOI of 50:1 with wild-type LVS, the FTL_0699::Tn5 mutant, the FTL_0724::Tn5 mutant, or the complemented mutant bacterium (FTL_0699::Tn5 + FTL_0699, FTL_0724::Tn5 + FTL_0724). (C and D) LPS-primed BMMϕ were challenged at an MOI of 50:1 with wild-type LVS, the FTL_0724::Tn5 mutant, or bacteria complemented with either FTL_0724 or catalytically inactive FTL_0724 R127A. IL-1β and IL-18 release into the supernatant 9 h postinfection was determined by ELISA. Determinations were performed in triplicate, and the results are expressed as means ± SD. Data are representative of the results of three independent experiments. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; N.S., nonsignificant.