Fig 4.

Intrinsic modification of a bacterial component was not responsible for the altered IL-1β secretion induced by the FTL_0699::Tn5 and FTL_0724::Tn5 mutants. LPS-primed BMMϕ were challenged with equivalent amounts of crude bacterial lysate (1 μg) (A) or purified bacterial DNA (750 ng) (B) from LVS, the FTL_0699::Tn5 mutant, or the FTL_0724::Tn5 mutant or the AIM2 agonist poly(dA-dT) in the presence of the Lipofectamine transfection agent. At 12 (A) or 6 (B) h postchallenge, IL-1β release into the supernatant was determined by ELISA. Determinations were performed in triplicate, and the results are expressed as means ± SD. Data are representative of the results of three independent experiments.