Table 2.
Primer | Sequence |
---|---|
crp KOa | |
Forward | GCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCTGTGTAGGCTGGAGCTGCTTCG |
Reverse | CGCGCTACCAGGTAACGCGCCACTCTGACGGGATTAACGACATATGAATATCCTCCTTAG |
crp KO confirmation | |
Forward | GTATGCAAAGGACGCCACAT |
Reverse | TTCGCCAAGCATTAACCCAA |
cyaA KO | |
Forward | GCGGAATCACAGTCATGACGGGTAGCAAATCAGGCGATACTGTGTAGGCTGGAGCTGCTTCG |
Reverse | TACTGCTGCAACAGCGGCGCGTCATGCTCCTGATTGGCAGCATATGAATATCCTCCTTAG |
cyaA KO confirmation | |
Forward | AACCAGGCGCGAAAAGTGGT |
Reverse | CTGAAAGGCGACGAGTGGAT |
otsBA KO | |
Forward | ATGTCTGTAAAGCGCGTTCTGCGCAACACAATAAGAAATGTGTAGGCTGGAGCTGCTTCG |
Reverse | CTACGCAAGCTTAGGAAAGGTAGCAACTTTATCGCGCTGCCATATGAATATCCTCCTTAG |
otsBA KO confirmation | |
Forward | AGCGAAACGCACTGTCTGAT |
Reverse | TTGCCTACGGTGAGTTAAGC |
dps KO | |
Forward | TTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGCACCAAACACCCCCCAAAACC |
Reverse | GTGATAGGAACAGCCAGAATAGCGGAACACATAGCTGGTGCTATACTTAGCACACAACCACACCACACCAC |
dps KO confirmation | |
Forward | GATAGCAGATGGATGCACTA |
Reverse | TGACAGTACGCAAAGAGAGC |
rpoS KO | |
Forward | CCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACCACCAAACACCCCCCAAAACC |
Reverse | TGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGACACACAACCACACCACACCAC |
rpoS KO confirmation | |
Forward | AATGATGATTGCCGAATGTGACGCTG |
Reverse | GCATTGTGTCGTTATGGGCGTAGG |
pcrp | |
Forward | CCCCC CTGCAG ATGGTGCTTGGCAAACCGCA |
Reverse | CCCCC GGTACC TTAACGAGTGCCGTAAACGA |
pcyaA | |
Forward | CCCCC CTGCAG TTGTACCTCTATATTGAGAC |
Reverse | CCCCC GGTACC TCACGAAAAATACTGCTGCA |
prpoS | |
Forward | CATTC CTGCAG ATGTTCCGTCAAGGGATCA |
Reverse | AGTGC AAGCTT TTATTCGCGGAACAGCGCT |
pRR48 sequencing primer | |
Forward | CTGCTGAAGAGTACTTTGG |
Reverse | CCAAAGCTGAAGACATCCAG |
KO, knockout.