Table 2.
Primer sequences
| Primer | Sequence |
|---|---|
| crp KOa | |
| Forward | GCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCTGTGTAGGCTGGAGCTGCTTCG |
| Reverse | CGCGCTACCAGGTAACGCGCCACTCTGACGGGATTAACGACATATGAATATCCTCCTTAG |
| crp KO confirmation | |
| Forward | GTATGCAAAGGACGCCACAT |
| Reverse | TTCGCCAAGCATTAACCCAA |
| cyaA KO | |
| Forward | GCGGAATCACAGTCATGACGGGTAGCAAATCAGGCGATACTGTGTAGGCTGGAGCTGCTTCG |
| Reverse | TACTGCTGCAACAGCGGCGCGTCATGCTCCTGATTGGCAGCATATGAATATCCTCCTTAG |
| cyaA KO confirmation | |
| Forward | AACCAGGCGCGAAAAGTGGT |
| Reverse | CTGAAAGGCGACGAGTGGAT |
| otsBA KO | |
| Forward | ATGTCTGTAAAGCGCGTTCTGCGCAACACAATAAGAAATGTGTAGGCTGGAGCTGCTTCG |
| Reverse | CTACGCAAGCTTAGGAAAGGTAGCAACTTTATCGCGCTGCCATATGAATATCCTCCTTAG |
| otsBA KO confirmation | |
| Forward | AGCGAAACGCACTGTCTGAT |
| Reverse | TTGCCTACGGTGAGTTAAGC |
| dps KO | |
| Forward | TTATTCGATGTTAGACTCGATAAACCACAGGAATTTATCCAGGTCGCGAGCACCAAACACCCCCCAAAACC |
| Reverse | GTGATAGGAACAGCCAGAATAGCGGAACACATAGCTGGTGCTATACTTAGCACACAACCACACCACACCAC |
| dps KO confirmation | |
| Forward | GATAGCAGATGGATGCACTA |
| Reverse | TGACAGTACGCAAAGAGAGC |
| rpoS KO | |
| Forward | CCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACCACCAAACACCCCCCAAAACC |
| Reverse | TGCCGCAGCGATAAATCGGCGGAACCAGGCTTTTGCTTGACACACAACCACACCACACCAC |
| rpoS KO confirmation | |
| Forward | AATGATGATTGCCGAATGTGACGCTG |
| Reverse | GCATTGTGTCGTTATGGGCGTAGG |
| pcrp | |
| Forward | CCCCC CTGCAG ATGGTGCTTGGCAAACCGCA |
| Reverse | CCCCC GGTACC TTAACGAGTGCCGTAAACGA |
| pcyaA | |
| Forward | CCCCC CTGCAG TTGTACCTCTATATTGAGAC |
| Reverse | CCCCC GGTACC TCACGAAAAATACTGCTGCA |
| prpoS | |
| Forward | CATTC CTGCAG ATGTTCCGTCAAGGGATCA |
| Reverse | AGTGC AAGCTT TTATTCGCGGAACAGCGCT |
| pRR48 sequencing primer | |
| Forward | CTGCTGAAGAGTACTTTGG |
| Reverse | CCAAAGCTGAAGACATCCAG |
a
KO, knockout.