Fig 11.
GST-Asp14 and GST-OmpA cooperatively block A. phagocytophilum infection of HL-60 cells. HL-60 cells were incubated with A. phagocytophilum (Ap) DC organisms in the presence of GST alone, GST-Asp14, GST-OmpA, GST-Asp14 and GST-OmpA, GST-Asp141–100 and GST-OmpA, or GST-Asp14 and GST-OmpA75–205 for 1 h. After removal of unbound organisms, host cells were incubated for 24 h and subsequently examined by confocal microscopy to assess the percentage of infected cells (A) or the mean number (±SD) of morulae per cell (B). Results shown are relative to GST-treated host cells and are the means ± SD for three to six experiments. Cells treated with either 4 μM GST-Asp14 or GST-OmpA were compared to cells treated with 4 μM GST, while cells treated with 4 μM GST-Asp14 and 4 μM GST-OmpA were compared to cells treated with 8 μM GST. Statistically significant (*, P < 0.05; **, P < 0.005; ***, P < 0.001) values are indicated.