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Asp14 colocalizes with confirmed outer membrane proteins. A. phagocytophilum-infected RF/6A cells were fixed and viewed by confocal microscopy to determine immunoreactivity with Asp14 antiserum in conjunction with antiserum against Msp2 (P44), Asp55, or VirB9, each of which is a confirmed outer membrane protein. Host cell nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole).
