Fig 1.

(A) Multiple alignment of selected fish IL-17A/F2 proteins with representative mammalian IL-17A and IL-17F molecules. Conserved residues are shaded using BOXSHADE (v3.21), with Cys residues indicated by black characters on a gray background. The predicted signal peptide (SP) is indicated by a black solid double-headed arrow above the alignment. The four cysteine residues that form two potential disulfide bonds are also indicated. Identical residues are identified by stars, whereas similar residues are identified by single dots. (B) Phylogenetic tree showing the relationship of the trout IL-17A/F2 amino acid sequence with those of other known IL-17 family members. The amino acid sequences were aligned using CLUSTAL W, and the tree was constructed by the N-J method supported with 10,000 bootstrap replications using MEGA 4.1 software. The trout IL-17A/F2 protein is in bold and underlined. Accession numbers of the IL-17 family members used are as follows: for IL-17A, human, NP_002181; cow, NP_001008412; mouse, NP_034682; rat, Q61453; and chicken, CAO79600; for IL-17F, human, NP_443104; cow, NP_001179011; mouse, NP_665855; rat, NP_001015011; and chicken, NP_989791; for IL-17A/F2, zebrafish, NP_00101863; fugu, BAI82579; and medaka, NP_001191713; for IL-17A/F1, fugu, BAI82578; medaka, NP_001191714; and zebrafish, NP_00101862; for IL-17A/F3, zebrafish, NP_00101862; medaka, NP_001191715; and fugu, BAI82580; for IL-17C, C1 trout, CAW30794; C2 trout, NP_001171959; zebrafish, NP_001018624; fugu, BAI82581; medaka, NP_001191723; human, NP_037410; cow, DAA20250; mouse, NP_665833; and rat, NP_0011780; for IL-17E, human, AAQ89484; mouse, NP_542767; cow, DAA25796; dog, XP_537375; and horse, XP_001918360; for IL-17B, mouse, NP_062381; rat, NP_446241; human, CAG33473; chicken, XP_425192; and xenopus, AAH75405; and for IL-17D, human, NP_612141; mouse, NP_665836; cow, DAA23895; medaka, NP_001191716; fugu, AAI58483; zebrafish, AAI62897; trout, CAE45584; and salmon, NP_001134365.