Fig 2.

(A) Multiple alignment of mammalian and fish IL-17RA protein sequences. Conserved residues are shaded using BOXSHADE (v3.21), with Cys residues indicated by black characters on a gray background. The predicted signal peptide (SP), D1 and D2 domains, transmembrane region (TM), SEFIR domain, and repeats (R) are indicated by black solid double-headed arrows above the alignment. The eight cysteine residues that form four potential disulfide bonds are also indicated. Identical residues are identified by stars, whereas similar residues are identified by single dots. (B) Phylogenetic tree showing the relationship of trout receptors with other known IL-17R family members. The amino acid sequences were aligned using CLUSTAL W, and the tree was constructed by the N-J method supported with 1,000 bootstrap replications using MEGA 4.1 software. The trout IL-17RA protein is in bold and underlined. Accession numbers of the sequences used in the analysis are as follows: for IL-17RA, salmon, NP_001158836; zebrafish, XP_001921444; chicken, XP_416389; human, CAJ86450; and mouse, NP_032385; for IL-17RD, zebrafish, AAI63933; xenopus, XP_002940076; chicken, NP_989846; human, NP_060033; and mouse, NP_602319; for IL-17RB, cow, AAI33637; human, NP_061195; mouse, NP_062529, and rat, NP_001100760; for IL-17RC, rat, NP_001164036; human, NP_116121; mouse, NP_598920; and cow, NP_001068646; and for IL-17RE, rat, NP_001004091; mouse, AAH69861; human, NP_705613; and cow, XP_001251391.