Fig 6.

Expression analysis of trout IL-17A/F2 and IL-17RA during a parasitic infection. Kidneys from rainbow trout infected with T. bryosalmonae or from unexposed fish (control) were collected during a natural infection. Kidney samples from control and parasite-infected fish exhibiting low kidney pathology (swelling indexes of 1 and 1-2) and advanced clinical pathology (swelling indexes of 2 and 3) were analyzed. RNA was extracted for real-time PCR analysis, and data were analyzed by the Pfaffl method (48) and expressed as fold change. Data represent averages + standard errors of the results (n = 5). Asterisks indicate significant differences (P < 0.05) relative to the control. Data were analyzed using the Student T test.