Table 1.
Oligonucleotide primers used for cloning and expression analysis
| Primer name | Primer sequence (5′ → 3′) | Application or reference |
|---|---|---|
| IL-17F1 | ACAGAGATCAGAGATGGAGC | Reverse transcription-PCR |
| IL-17R1 | CATATGTCAATGGGGATCTT | |
| F1 | ACAGAGATCAGAGATGGAGCTCAAAAGC | Primers used to obtain trout IL-17A/F2 |
| R1 | CCTCTGATTCCTCTGTGGGAATCTTG | |
| F2 | GAACATTCACACACGCTCTCTGTCAC | |
| R2 | CATATGTCAATGGGGATCTTTTTCTTGAA | |
| F3 | GCTACAGCGCCTCCTTCCTGTCT | 3′-RACE |
| F4 | GCCTGCAGTACACTACATCTATGGCCTT | |
| IL-17-RF2 | GGATCCaAAAGGAATGAAGGTGACAAAGG | Recombinant protein production |
| IL-17-RR3 | CTACATCAAGTAGTCCTTGCCCA | |
| EF-1α F | CAAGGATATCCGTCGTGGCA | Real-time PCR |
| EF-1α R | ACAGCGAAACGACCAAGAGG | |
| IL-17A/F2 F | CGTGTCGAAGTACCTGGTTGTGT | Real-time PCR |
| IL-17A/F2 R | GGTTCTCCACTGTAGTGCTTTTCCA | |
| IL-17RA F | CGTAATCCAGCATATGAAAAGTGGTCTT | Real-time PCR |
| IL-17RA R | CTCCAAACACACCTTGCTCCTCT | |
| BD-3 F | GCTTGTGGAATACAAGAGTCATCTGC | Real-time PCR (37) |
| BD-3 R | GCATACATTCGGCCATGTACATCC | |
| IL-6 F | CCTTGCGGAACCAACAGTTTG | Real-time PCR |
| IL-6 R | CCTCAGCAACCTTCATCTGGTC | |
| IL-8 F | AGAATGTCAGCCAGCCTTGT | Real-time PCR |
| IL-8 R | TCTCAGACTCATCCCCTCAGT | |
| TNF-α1 F | GTGGGGTCCTCTTAATAGCAGGTC | Real-time PCR (38) |
| TNF-α1 R | ATTTCATCCTGCATCGTTGACG |
a
A BamHI restriction site is underlined.