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GXM dampens TNF-α production in spleens, sera, and lymph nodes of LPS-treated mice. Mice treated as described in Materials and Methods were sacrificed 24 h after LPS treatment. TNF-α levels in spleen supernatant fluids (A), sera (B), and lymph node supernatant fluids (C) were determined by ELISA. Values represent the means and SEM for five separate experiments. *, P < 0.05 (LPS-plus-GXM-treated or wortmannin-treated mice versus LPS-treated mice).
