Fig 1.

Virulence of wild-type B. cenocepacia H111 and auxotrophic mutants in the C. elegans model. C. elegans were counted 48 h after spreading the worms on medium covered with a lawn of the respective bacterial strain. Each experiment consisted of three independent replicates. Black bar represents the wild type (wt) (H111), dark gray bars represent mutants grown on NG medium, and light gray bars represent mutants grown on nutritionally supplemented NG medium. Nutritional supplementation was as follows: hisG and hisH, histidine; cysI and cysB, cysteine, trpA, trpB, and trpF, dl-tryptophan; purD, purF, purL, and purA, adenosine.