Fig 2.

Siderophore production of attenuated B. cenocepacia H111 Tn5 mutants. (A) Mutants were inoculated on CAS plates and incubated at 37°C for 48 h. The halo diameter corresponds to siderophore activity. A pink-tinted halo is indicative of pyochelin production. (B) Production of pyochelin as determined by thin-layer chromatography. Bands corresponding to pyochelins I and II (P1/P2) and salicylate (SA) are indicated. Extracts were as follows: SA, salicylate standard; wt, wild-type H111; cysI, H111 cysI; aroK, H111 aroK; cepR, H111 cepR.