Fig 6.
Virulence of wild-type B. cenocepacia H111type, attenuated auxotrophic Tn5 mutants, and complemented mutant strains in the G. mellonella infection model. G. mellonella larvae were infected with approximately 2 × 105 bacteria and incubated at 30°C in the dark. Live and dead larvae were counted after 20, 24, 40, 48, and 72 h postinfection. The curves were calculated using three independent replicates. The significance of the difference between results for the wild type and the Tn5 mutants was determined using the log rank (Mantel-Cox) test and is indicated as follows: *, 0.01 ≤ P ≤ 0.05; **, 0.001 ≤ P ≤ 0.01; ***, P < 0.001.