Table 3.
B. cenocepacia H111 mutants attenuated in C. elegans
| Strain | Gene disrupted | H111 gene ID | Mean (SD) % dead C. elegans larvae after 48 ha | D. melanogaster survival time (h) | Mean (SD) % surviving G. mellonella after 48 h | AHL productiona | Protease activitya | EPS productiona | Siderophore productiona | Expression of AidAa | Growth on ABCa |
|---|---|---|---|---|---|---|---|---|---|---|---|
| HIII | None (wt) | 89 (4) | <72 | 10 (10) | + | + | + | + | + | + | |
| A4 | purD | 358071918 | 24 (4) | >336 | 72 (18) | < | < | − | − | + | − |
| D9 | purF | 358073772 | 44 (10) | >336 | 29 (8) | + | < | < | + | + | − |
| C2-1 | purL | 358073038 | 30 (8) | >336 | 50 (20) | < | + | − | − | + | − |
| F8 | purA | 358073158 | 29 (8) | <72 | 0 (0) | + | + | < | < | + | − |
| A11 | aroK | 358076029 | 25 (4) | <288 | 93 (12) | + | + | − | +c | + | − |
| H2 | ilvC | 358071882 | 21 (9) | >336 | 27 (25) | + | + | < | − | + | − |
| C5 | cysI | 358073512 | 29 (8) | <72 | NTd | + | + | < | +c | + | − |
| E10 | cysI | 358073512 | 41 (10) | <72 | NT | + | + | < | +c | + | − |
| F7 | cysI | 358073512 | 30 (16) | <72 | NT | + | + | < | +c | + | − |
| B1 | cysI | 358073512 | 25 (9) | <72 | NT | + | + | < | +c | + | − |
| G11 | cysB | 358073513 | 62 (8) | <72 | NT | + | + | + | +c | + | + |
| E11 | ahcY | 358071424 | 11 (9) | <72 | NT | + | + | − | + | + | − |
| E8 | hisG | 358075994 | 44 (12) | <72 | NT | + | + | < | + | + | − |
| G5-1 | hisH | 358075989 | 27 (3) | <72 | NT | + | + | < | < | + | < |
| D1 | trpA | 358073777 | 43 (9) | <72 | NT | + | + | − | + | − | − |
| G5-2 | trpB | 358073779 | 45 (12) | <72 | NT | + | + | + | + | < | − |
| B8 | trpF | 358073780 | 33 (10) | <72 | NT | < | + | − | + | + | − |
| C2-2 | gatA | 358076086 | 63 (15) | <72 | NT | + | < | + | + | + | + |
| E5 | rsuA | 358074812 | 29 (9) | <72 | NT | < | − | + | − | < | + |
| G3 | lon | 358073031 | 46 (10) | <72 | NT | < | − | + | + | + | + |
| E12-2 | rsaM | 358072205 | 17 (8) | <72 | NT | + | < | + | < | < | + |
| D6b | pyrD | 358074754 | 23 (7) | >336 | 87 (15) | < | − | − | − | + | − |
| G1 | cepR | 358072206 | 7 (5) | <72 | 0 (0) | − | − | < | + | + | + |
a
+, wt level; <, less than wt level; −, absence.
b
This strain showed a reduced growth rate in LB medium (see Fig. S1 in the supplemental material).
c
Halo on CAS agar of normal size but pink color absent.
d
NT, not tested.