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Native PAGE and SDS-PAGE profiles of wild-type and six-Asp mutant rPA after storage for various periods of time. Equal amounts of rPA samples that had been stored for up to 4 weeks at 25°C as described in Materials and Methods were analyzed on native PAGE and SDS-PAGE gels. (A) Wild-type rPA, native PAGE; (B) six-Asp mutant rPA, native PAGE; (C) wild-type rPA, SDS-PAGE; (D) six-Asp mutant rPA, SDS-PAGE. Lanes are marked with time of storage (in weeks). Lane M, molecular weight markers.
