Table 2.
Primers used in quantitative reverse transcriptase PCR
| Gene | Primer sequence |
|
|---|---|---|
| Forward | Reverse | |
| crp | CCGTCAGGAAATCGGTCAGA | TGCGTCCCACGGTTTCA |
| fimH | GATGCGGGCAACTCGATT | GCCCTGCGCTGGTGAA |
| fimA | TGCGGGTAGCGCAACAA | |
| fimB | GGAGATTCATCCGCACATGTT | TCCCATATTCGCCAAAGCA |
| fimC | CGTTGCCACCCGATCAG | AATTCGCGCTACGACGAAA |
| fimD | CAGTGCCAGCTACAGCATGTC | TACCGGCCAGATTGGTCATC |
| fimE | CGCGGGAGTCGGCTTT | ACCGGCATCACGAATAATGC |
| fimF | CCCTGTGGTAACGCCGTTT | GGCTATCTGCAACGCCAGTAA |
| fimG | AATCAGCGCACTTCCCGTTA | TGAGTGGCTCCGCCATTTAC |
| fimH | GATGCGGGCAACTCGATT | GCCCTGCGCTGGTGAA |
| fimI | CGGAAACTTGCCGGATTG | GCCCCATATTGACCGTCATT |
| cfaA | CAGGAGCGAGTAAATCAATACGTTT | CTATCTGGTTTTACCGCCTCAAA |
| cfaB | AAATGGCGTATCATCTTCTCAAGAG | GGCGGTACCGGCAGTTT |
| cfaC | GGTTGGAGTGGATGCTACGAA | CAGAGTACTTGTCCATCCTAATAAAGGA |
| eatA | AAAGCTCCAATGCCTGATTTTAGTA | GTGTATGGTCTCCTGGTGGTAATG |
| etpB | GAACAGGGATAATGCCACAAA | TCCCAGATTCACCTCGTTTT |
| etpA | ATGCCGGTGGCTATATTGTG | GTACGGATGGTGCCACTGTT |
| etpC | CCACCTGGTCTGAGCATATTG | CCGGGTCTCCCAGGTAGT |
| eaeH | GAAGGATGCGTACGGGAAC | CCGCTAAACACTGGTGCAT |
| tia | ACAGGCTTTTATGTGACCGGTAA | GACGGAAGCGCTGGTCAGT |
| arcA | ATCAATCTGCCGGGTAAGAACGGT | TCCAGATCACCGCAGAAGCGATAA |
| cexE | GCGTGTAATGAGACAGTTGCTAAAG | CCTCATAATTTACAGTCCGATGCA |
| aatD | CAAAACCACGGAGGAACTGTACT | GCGATGTTGCGAATACGCTAT |
| aatP | CAACTTTTTTGGATAGCCTTGGA | GCGCCGTTTCTAATGGGATA |
| aatB | CAGCGGTATGGAGGGTGTAAA | AGATCTGATGATAGGTTTCCATTGTTT |
| aatA | CTTGAGGAGGCGAATATTAATTTTGA | GGCGTCAGCCCCAAAGA |
| aatC | TGGCATATCTCCAGAAGCATCA | CTTGCAAGCGCAACTCGTT |
| gspC | GTCGCCACGCCGGTAA | GACGCGTTTCTGCCACAGA |
| gspD | CGGATACCAACGGCGATCT | CCGCTAAAGCCGGAAAGAA |
| gspE | TGCGCGCGATTTTGC | CGGATTTCACCGACCATCA |
| gspF | AAAGAGCTTATCCCCGTGCAT | AACATCCCCCCTGACGAAGT |
| gspG | GCAAGTCTGGTGGTGCCTAAC | TTTTGCCGATCCGCTTTCT |
| gspH | CAGATCCGTTTTTCGCCTTTT | CGAGTAGAAGCGCAGCGTAA |
| gspI | GCTCCAGGCGGTGGATATT | TGACTGAATGACCGACGAAAAG |
| gspJ | GCCGACAATGCAAAAGTTGA | CCGTCGTAGAACTGCAAACG |
| gspK | TGCTGGTAACCATCACGCTTT | GCGTTCGCCCAAGTTGTT |
| gspL | CTCCAGCAATGGCTGCAA | GATCCATTCGCCGGGAAT |
| gspM | CGCTGCGGGTGACACAA | ACATTCACCATCCCAGTCTTCTC |
| eltA | TTCATCAAGAACAATTACAGGTGATA | TGATATTTCCTGAGATATATTGTGCTC |
| yebT | TCGTAAACTCACCAGCAAAGG | AGAAACTCAACGCCATCCAG |