Table 3.

Alteration of crp- and cAMP-CRP-dependent genes on cell contact^a

Gene name	Protein annotation	Time (min)	Fold change (log2)b	P value
crp	cAMP regulatory protein	30	−1.60	0.019
		60	−1.84	0.036
		120	−2.16	0.038
Flagellar genes
flgK	Flagellar hook-associated protein 1	30	−1.22	0.042
		60	−	NSc
		120		NS
fliD	Flagellar hook-associated protein 2	30	−1.169	0.015
		60		NS
		120	−1.106	0.0017
flit	Flagellar biosynthesis protein	30		NS
		60		NS
		120	−1.12	0.028
flgL	Flagellar hook-associated protein 3	30	−1.55	0.017
		60		NS
		120	−1.44	0.016
fliC	Flagellin	30	−3.19	0.02
		60	−3.33	0.0006
		120	−2.61	0.0019
flgM	Anti-sigma 28 factor	30	−1.096	0.0082
		60	−1.488	0.0310
		120		NS
cheY	Chemotaxis regulatory protein	30	−2.894	0.0495
		60	−2.979	0.0004
		120	−2.379	0.0051
cheW	Purine-binding chemotaxis protein	30		NS
		60	−1.176	0.0011
		120		NS
luxS	S-Ribosylhomocysteine lyase	30		NS
		60	−2.3	0.0003
		120	−1.93	0.016
cooA	CS1 type fimbrial major subunit	30	−3.008	0.0241
		60	−3.212	0.0001
		120	−4.580	0.0043
ompA	Outer membrane protein A	30	−3.137	0.0106
		60	−2.71	0.0040
		120	−2.64	0.016
tolC	TolC outer membrane channel protein	30	−1.74	0.022
		60	−1.8267	0.013
		120		NS
eltA	Heat-labile toxin, A subunit	30		NS
		60	−1.19	0.017
		120	−2.46	0.003
ST-1b	Heat-stable toxin (ST-H)	30	−5.14	0.036
		60	−2.79	0.015
		120		NS

^aThe data reported in the table depict all genes with previously established CRP-cAMP dependence for which there were statistically significant changes in gene expression comparing attached and planktonic organisms using the FDA-E. coli-Shigella (FDA-ECSG) Affymetrix array.

^bAdherent versus planktonic (log₂ values).

^cNS, nonsignificant change.