Fig 3.

Depletion of PMNs allows the yopK mutant to spread systemically. Real-time monitoring of Y. pseudotuberculosis infection using in vivo imaging. PMN-depleted mice treated intraperitoneally with α-Ly6G/6C and control mice receiving PBS were orally infected with 1.2 × 10⁸ wild-type bacteria [YPIII(pCD1, Xen4)] or 1.3 × 10⁸ yopK mutant [YPIII(pCD155, Xen4)] bacterial strains. (A) Bioluminescent signals from the infected animals on the indicated days postinfection are presented as photons/second. The data were compared by using the Student t test; differences were considered significant at a P value of <0.05 (*, P < 0.05; **, P < 0.01). (B) Bioluminescent signals from a representative set of dissected organs for each bacterial strain on days 1, 3, 5, and 7 p.i. Beneath each organ image is a summary presenting the number of each indicated organ with signals/all dissected organs. PP, Peyer's patches; M, mesenteric lymph node (MLN); L, liver; S, spleen.