Table 1.
Bacterial strains and their relevant characteristics
| Strain | Relevant characteristics | Reference |
|---|---|---|
| N. gonorrhoeae strains | ||
| FA19 | PorB.1A (serotype PIA-1), L8 LOS, binds C4BP, binds fH | 24 |
| FA19 lptA::spc | PorB.1A, L8 LOS; lptA::spc | 22 |
| FA19 lptA::spc lptA+ | PorB.1A, L8 LOS; lptA::spc lptA+ (FA19 lptA::spc complemented with lptA driven from an IPTG-inducible promoter) | 22 |
| 273 | PorB.1A (serotype PIA-6), LNT (3F11) LOS, binds C4BP, binds fH | 17 |
| 273 lptA::spc | PorB.1A, LNT (3F11) LOS; lptA::spc | This work |
| 252 | PorB.1A (serotype PIA-4), LNT (3F11) LOS, does not bind C4BP, binds fH | 17 |
| 252 lptA::spc | PorB.1A, LNT (3F11) LOS; lptA::spc | This work |
| FA1090 | PorB.1B (serotype PIB-3a), LNT (3F11) LOS, binds C4BP, binds fH | 25 |
| FA1090 lptA::spc | PorB.1B, LNT (3F11) LOS; lptA::spc | This work |
| 1291 | PorB.1B (serotype PIB-3a), LNT (3F11) LOS, binds C4BP, binds fH | 26 |
| 1291 lptA::spc | PorB.1B, LNT (3F11) LOS; lptA::spc | This work |
| N. meningitidis strains | ||
| H44/76 cap− | Unencapsulated; siaD::cat | 29 |
| H44/76 cap− fHbp | Unencapsulated; siaD::cat fhbp::ermC | 29 |
| H44/76 cap− lptA::spc | Unencapsulated; siaD::cat lptA::spc | This work |
| H44/76 cap− fHbp lptA::spc | Unencapsulated; siaD::cat fhbp::ermC lptA::spc | This work |
| Y2220 cap− lst | Unencapsulated, carries insertional inactivation of LOS sialyltransferase; siaD::cat lst::kan | 30 |
| Y2220 cap− lst lptA::spc | Unencapsulated, carries insertional inactivation of LOS sialyltransferase; siaD::cat lst::kan lptA::spc | This work |
| Y2220 cap− lst PorFA19 | Unencapsulated, carries insertional inactivation of LOS sialyltransferase, has Y2220 PorB2 replaced with FA19 PorB.1A, binds C4BP and fH; siaD::cat lst::kan porB.1AFA19 | 31 |
| Y2220 cap− lst PorFA19 lptA::spc | Unencapsulated, carries insertional inactivation of LOS sialyltransferase, has Y2220 PorB2 replaced with FA19 PorB.1A; binds C4BP and fH; siaD::cat lst::kan porB.1AFA19 lptA::spc | This work |
a
The FA1090 and 1291 PorB molecules are 97% identical, with 11 amino acid differences in 348 amino acids.