Fig 3.

Normalized CFU counts, percentages of intact cells, and fatty acid titers after 8 h for double efflux pump deletions in strain TY06 (where shown; BTE-H204A) and TY05 (BTE). Cultures were sampled at 8 h postinoculation. (a) Normalized CFU counts (number of CFU per milliliter from plate counts divided by the number of cells per milliliter from flow cytometry counts). (b) Percentages of intact cells determined by SYTOX Green flow cytometry assay. TY05 and TY05 ΔacrAB were independently run as negative controls. (c) Total fatty acid titers for double efflux pump deletions in TY05 at 8 h postinoculation. TY05 ΔacrAB ΔemrAB exhibits much lower fatty acid production (primarily reduced C₈ to C₁₄) than other TY05 strains. Titers lower than those of other TY05 strains persist in TY05 ΔacrAB ΔemrAB after 24 h (see Fig. S6 in the supplemental material). #, data not measured.