Table 2.

Bacterial strains and plasmids used in this study^a

Strain or plasmid	Relevant genotype/propertyb	Source or reference
Strains
TY05	K-12 MG1655 fadD::Ptrc-BTE fadE::Ptrc-BTE fadAB::Ptrc-BTE	34
TY06	K-12 MG1655 fadD::Ptrc-BTE-H204A fadE::Ptrc-BTE-H204A fadAB::Ptrc-BTE-H204A	34
TY05ara	K-12 MG1655 fadD::Ptrc-BTE fadE::Ptrc-BTE fadAB::Ptrc-BTE ΔaraFGH Φ(ΔaraEp PCP18-araE) ΔaraBAD	This work
BW25113	lacIq rrnB3 F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 hsdR514	36
JW5249-1	BW25113 ΔmarA752::kan	36
JW4359-1	BW25113 Δrob721::kan	36
JW4023-5	BW25113 ΔsoxS756::kan	36
JW5503-1	BW25113 ΔtolC732::kan	36
JW2341-1	BW25113 ΔfadL752::kan	36
JW1819-1	BW25113 Δprc755::kan	36
JW2454-1	BW25113 ΔacrD790::kan	36
JW1040-1	BW25113 ΔmdtG723::kan	36
JW1655-1	BW25113 ΔmdtK740::kan	36
JW0826-1	BW25113 Δcmr742::kan	36
JW0912-1	BW25113 ΔompF746::kan	36
DY330	K-12 W3110 ΔlacU169 gal490 pglΔ8 λ cI857 Δ(cro-bioA) (Tetr)	39
Plasmids
pBAD33	PBAD promoter, pACYC origin, Cmr	44
pBAD33*	pBAD33 with araC-C280* mutation	45
pBAD33*-acrAB	pBAD33* carrying acrAB under PBAD control	This work
pBAD33*-mdtEF	pBAD33* carrying mdtEF under PBAD control	This work
pBAD33*-mdtEF-tolC	pBAD33* carrying mdtEF-tolC artificial operon under PBAD control	This work
pBAD33*-emrAB	pBAD33* carrying emrAB under PBAD control	This work
pBAD33*-mdtABCD	pBAD33* carrying mdtABCD under PBAD control	This work
pKD13	Template plasmid, R6K gamma origin, Ampr Kanr	38
pCP20	Carries yeast FLP recombinase under constitutive promoter control, pSC101 origin, λ cI857+, λ pR Rep(Ts), Ampr Cmr	41

^aFor the full strain and plasmid list, see Table S2 in the supplemental material.

^bAbbreviations: Tet^r, tetracycline resistance; Amp^r, ampicillin resistance; Cm^r, chloramphenicol resistance; Kan^r, kanamycin resistance; Ts, temperature sensitive.