Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype/genetic markers | Source or reference |
|---|---|---|
| E. coli strains | ||
| W3110 | Wild type, F− λ− | E. coli Genetic Stock Center, Yale University |
| W3110F | W3110, ΔpspF739::Kanr | This work |
| BC202 | W3110, ΔyqjA::Tetr ΔyghB781::Kanr | 4 |
| BC203 | W3110, ΔyqjA::Tetr | 4 |
| BC204 | W3110, ΔyghB781::Kanr | 4 |
| BC202C | W3110, ΔyqjA::Tetr ΔcpxR772 ΔyghB781::Kanr | This work |
| BC202F | W3110, ΔyqjA::Tetr ΔpspF739 ΔyghB781::Kanr | This work |
| BC202R | W3110, ΔyqjA::Tetr ΔrcsB770 ΔyghB781::Kanr | This work |
| BC202bae | W3110, ΔyqjA::Tetr ΔbaeR778 ΔyghB781::Kanr | This work |
| JW2976 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− ΔyghB781::Kanr rph-1 Δ(rhaD-rhaB)568 hsdR514 | 3 |
| JW3883 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 ΔcpxR772::Kanr hsdR514 | 3 |
| JW1296 | F− Δ(araD-araB)567 ΔpspF739::Kanr ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 hsdR514 | 3 |
| JW2205 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− ΔrcsB770::Kanr rph-1 Δ(rhaD-rhaB)568 hsdR514 | 3 |
| JW0192 | F− Δ(araD-araB)567 ΔrcsF721::Kanr ΔlacZ4787(::rrnB-3) λ− rph-1 Δ(rhaD-rhaB)568 hsdR514 | 3 |
| JW2064 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ− ΔbaeR778::Kanr rph-1 Δ(rhaD-rhaB)568 hsdR514 | 3 |
| TB28 | MG1655, ΔlacIZYA | 45 |
| RS28A | TB28, ΔyqjA::Tetr | This work |
| RS28B | TB28, ΔyghB781::Kanr | This work |
| RS28B* | TB28, ΔyghB781 | This work |
| RS28C | TB28, ΔcpxR772::Kanr | This work |
| RS28F | TB28, ΔpspF739::Kanr | This work |
| RS28R | TB28, ΔrcsB770::Kanr | This work |
| RS28bae | TB28, ΔbaeR778::Kanr | This work |
| RS28AB | TB28, ΔyqjA::Tetr ΔyghB781::Kanr | This work |
| RS28ABC | TB28, ΔyqjA::Tetr ΔcpxR772::Kanr ΔyghB781 | This work |
| RS28ABF | TB28, ΔyqjA::Tetr ΔpspF739::Kanr ΔyghB781 | This work |
| RS28ABR | TB28, ΔyqjA::Tetr ΔrcsB770::Kanr ΔyghB781 | This work |
| RS28ABrcsF | TB28, ΔyqjA::Tetr ΔrcsF721::Kanr ΔyghB781 | This work |
| RS28ABbae | TB28, ΔyqjA::Tetr ΔbaeR778::Kanr ΔyghB781 | This work |
| MVA4 | MC1061 ϕ(pspA-lacZ) (Ampr) | 46 |
| RSP28 | TB28 ϕ(pspA-lacZ) (Ampr) | This work |
| RSP28A | RS28A ϕ(pspA-lacZ) (Ampr) | This work |
| RSP28B | RS28B ϕ(pspA-lacZ) (Ampr) | This work |
| RSP28F | RS28F ϕ(pspA-lacZ) (Ampr) | This work |
| RSP28AB | RS28AB ϕ(pspA-lacZ) (Ampr) | This work |
| RSP28ABF | RS28ABF ϕ(pspA-lacZ) (Ampr) | This work |
| TB28sc | TB28; pSH10; pRSpssAC | This work |
| RS28ABsc | RS28AB; pSH10; pRSpssAC | This work |
| Plasmids | ||
| pBADHisA | Expression vector; araBAD promoter, Ampr | Invitrogen |
| pBADCamHisA | Expression vector; araBAD promoter, Ampr Camr | This work |
| pACYC184 | Low-copy-number expression vector; Tetr Kanr | New England Biolabs |
| pRSpssAC | H6-pssA expression vector; araBAD promoter, Ampr | This work |
| pRS101 | σ32-responsive vector; Camr, pACYC184 (grpE promoter-lacZ) | This work |
| pRS102 | σE-responsive vector; Camr, pACYC184 (degP promoter-lacZ) | This work |
| pRS103 | RcsB-responsive vector; Camr, pACYC184 (rprA promoter-lacZ) | This work |
| pRS104 | BaeR-responsive vector; Camr, pACYC184 (mdtA promoter-lacZ) | This work |
| pBADYqjA | H6-yqjA expression vector; araBAD promoter, Ampr | 7 |
| pBADMdfA | H6-mdfA expression vector; araBAD promoter, Ampr | This work |
| pBADTatABC | tatABC operon expression vector; araBAD promoter, Ampr | 5 |
| pSH10 | CpxR-responsive vector; Ampr, pFZY1 (cpxP promoter-lacZ) | 47 |
| pCP20 | FLP+, λ cI857+, λ ρR Repts Ampr Camr | 48 |