Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| Streptococcus mutans | ||
| UA159 | Wild type, serotype c | ATCC 700610 |
| GMS584 | UA159 derived, sloR deficient, Emr | 24 |
| GMS182 | GMS584 transformed with pJRH182, Emr Kmr | This study |
| GMS186 | GMS584 transformed with pJRH186, Emr Kmr | This study |
| Escherichia coli | ||
| DH5α | F− supE44 ΔlacU169 ϕ80dlacZΔM15 hsdR17 recA1 endA1 gyrA96 thi-1 relA | Bethesda Research Laboratories, Gaithersburg, MD |
| XL10 Gold | Tetr Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac hte [F′ proAB lacIqZΔM15Tn10 (Tetr) Amy Camr] | Stratagene |
| Plasmids | ||
| pES-03 | pET101/D-TOPO (Invitrogen) derivative expressing wild-type SloR, Ampr | This study |
| pJL84 | Promoterless chloramphenicol acetyltransferase (cat) reporter plasmid, Kmr Emr | 39 |
| pJRH182 | pJL84 derivative containing a PsloABC-cat fusion | This study |
| pJRH186 | pJL84 derivative containing a PsloR-cat fusion | This study |
| pDL277 | E. coli-streptococcal shuttle vector, Spr | 20 |
| pJRH277 | pDL277 derived, harbors wild-type sloR gene, Spr | This study |
| pJRH277D7A | pJRH277 derivative expressing SloR(D7A) | This study |
| pJRH277S34A | pJRH277 derivative expressing SloR(S34A) | This study |
| pJRH277P36R | pJRH277 derivative expressing SloR(P36R) | This study |
| pJRH277S39A | pJRH277 derivative expressing SloR(S39A) | This study |
| pJRH277E40A | pJRH277 derivative expressing SloR(E40A) | This study |
| pJRH277S34A/S39A/M41V | pJRH277 derivative expressing SloR(S34A/S39A/M41V) | This study |
| pJRH277S34A/P36R/S39A/E40A | pJRH277 derivative expressing SloR(S34A/P36R/S39A/E40A) | This study |
| pJRH277H76A | pJRH277 derivative expressing SloR(H76A) | This study |
| pJRH277E80A | pJRH277 derivative expressing SloR(E80A) | This study |
| pJRH277E99A | pJRH277 derivative expressing SloR(E99A) | This study |
| pJRH277E102A | pJRH277 derivative expressing SloR(E102A) | This study |
| pJRH277H103A | pJRH277 derivative expressing SloR(H103A) | This study |
| pJRH277E99A/E102A/H103A | pJRH277 derivative expressing SloR(E99A/E102A/H103A) | This study |
| pJRH277C123A | pJRH277 derivative expressing SloR(C123A) | This study |
| pJRH277H125A | pJRH277 derivative expressing SloR(H125A) | This study |
| pJRH277D160A | pJRH277 derivative expressing SloR(D160A) | This study |
| pJRH277E169A | pJRH277 derivative expressing SloR(E169A) | This study |
| pJRH277Q170A | pJRH277 derivative expressing SloR(Q170A) | This study |
| pJRH277E169A/Q170A | pJRH277 derivative expressing SloR(E169A/Q170A) | This study |
| pJRH277L179Q | pJRH277 derivative expressing SloR(L179Q) | This study |
| pJRH277ΔR141-Y217 | pDL277 derivative expressing SloR(ΔR141-Y217) | This study |
| pJRH277ΔQ170-Y217 | pDL277 derivative expressing SloR(ΔQ170-Y217) | This study |
| pJRH277ΔL181-Y217 | pDL277 derivative expressing SloR(ΔL181-Y217) | This study |
a
Emr, erythromycin resistant; Kmr, kanamycin resistant; Ampr, ampicillin resistant; Spr, spectinomycin resistant.