Fig 1.

Identification of influenza A (H1N1) virus of porcine origin in a pig farm employee by RT-PCR versus culture (see Fig. 2). A nasopharyngeal specimen from a farm employee with an influenza-like illness was screened for influenza virus by using a panel of specific RT-PCR assays. A generic RT-PCR used to screen animal and human matrix gene sequences of influenza A viruses (5) provided a signal (shown are the amplification and multicomponent plots) considered to represent ambiguous or even negative results, and all other combinations targeting the hemagglutinin genes of human viruses (seasonal H1, H1 2009, and H3) (5, 6) remained negative (data not shown). ΔRn, delta of the normalized reporter value.