Table 2.
In silico and in vitro Tm analysis of the 189-bp amplicon for 12 blaKPC alleles
| KPC | Nucleotide at positiona: |
Tm (°C) |
|||
|---|---|---|---|---|---|
| 716 | 814 | 843 | Predictedb | Actualc | |
| KPC-2 | T | C | A | 87.6 | 87.2 |
| KPC-3 | T | T | A | 87.3 | 86.9 |
| KPC-4 | G | C | A | 87.7 | ND |
| KPC-5 | T | C | A | 87.6 | 87.2 |
| KPC-6 | G | C | A | 87.7 | ND |
| KPC-7 | T | T | A | 87.3 | ND |
| KPC-8 | G | T | A | 87.3 | ND |
| KPC-9 | C | T | A | 87.6 | ND |
| KPC-10 | T | T | A | 87.3 | ND |
| KPC-11 | T | C | A | 87.6 | ND |
| KPC-12 | T | C | A | 87.6 | ND |
| KPC-13 | T | T | G | 87.4 | ND |
a
The nucleotide position in comparison to GenBank accession number EU176012.
b
The predicted Tm using a web-based oligonucleotide calculator (http://www.basic.northwestern.edu/biotools/oligocalc.html).
c
The Tm observed in this assay. ND, not determined.