Table 3.

Sensitivity and specificity of HRM and ScreenClust analyses for the presence of bla_KPC and differentiation of bla_KPC-2-like and bla_KPC-3-like gene variants

Parameter	Presence of blaKPCa	Variantsb
		blaKPC-2-like	blaKPC-3-like
Tm ± SD (°C)	87 ± 0.17	87.2 ± 0.03	86.8 ± 0.03
No. of tested strains	66	14	17
Sensitivity (%)	66/66 (100)	14/14 (100)	17/17 (100)
Specificity (%)	73/73 (100)	17/17 (100)	14/14 (100)

^aCompared to previous characterization by endpoint PCR.

^bCompared to direct sequencing of bla_KPC gene in a subset of 31 isolates.