Table 1.
Broad-range primers and targets for PCR/ESI-MS BFA
| Target genea | Primer sequence (5′ → 3′) | Locus | Amplicon size (bp) | Range of targeted fungi |
|---|---|---|---|---|
| LSU rRNA | TCATTTTTGGTAAGCAGAACTGGCGA | 5186 | 107–108 | Broad |
| TGTCTAGATGAACTAACACCTTTTGTGGT | ||||
| LSU rRNA | TGCCGAAGTTTCCCTCAGGATAGCA | 5187 | 72–78 | Broad |
| TCAAGGCCTCTAATCATTCGCTTTACCTC | ||||
| SSU rRNA | TGCAGGCCTATGCTCGAATACATTAGCATTGTCCCTATTAATCATTACGGCGGTCCTA | 5178 | 99–101 | Ascomycetes and basidiomycetes |
| LSU rRNA | TGTGAAGCGGCAAAAGCTCAAATTT | 3030 | 116–130 | Ascomycetes and basidiomycetes |
| TTCTCACCCTCTGTGACGGCCTGTTCC | ||||
| LSU rRNA | TGGAGTCTAACATCTATGCGAGTGTT | 5185 | 137–153 | Ascomycetes and some basidiomycetes |
| TCCCACAGCTATGCTCTTACTCAAATCC | ||||
| LSU rRNA | TTGTGTAGAATAGGTGGGAGCTTCGGC | 3766 | 148–154 | Ascomycetes and basidiomycetes |
| TCTGACAATGTCTTCAACCCGGATC | ||||
| mtSSU rRNA | TCCAAATTACGTGCCAGCAGTCG | 5181 | 90 | Ascomycetes (except Candida spp) and zygomycetes |
| TCCCTACCGTCTAGGTACCCTTTAAACC | ||||
| SSU rRNA | TCTTGGATTGACCGAAGACAAACTACTG | 4836 | 136–136 | Most ascomycetes and basidiomycetes, zygomycetes |
| TCTCTAGTCGGCATCGTTTGTGGTTAAG | ||||
| tub | TGCCGCGGTGCTCACTCTTTC | 4837 | 109 | Most ascomycetes (except Candida spp.) |
| TGTATCGGCCGTTGCGGAAGTC | ||||
| mt cytB | TCCCCGTAACAATTTTCTTATTCTTCTTAGTATTAGTGCATTGGGTTAGCTGGAATATAATTATCAGGATG | 5172 | 106 | Zygomycetes |
| SSU rRNA | TCGAAGACGATTAGATACCGTCGTAGTC TCAGAGCCCAAAAACTTTACTTTCGCTAAG | 5174 | 113–116 | Zygomycetes |
| mtSSU rRNA | TCCAAGTACTTGACACATGCTAATCG | 3862 | 102–133 | Eurotiales (Aspergillus, Penicillium) |
| TCCCCTTACTTTAAGGTAGCCAAATTATC | ||||
| mtSSU rRNA | TGGTACAGTGGAGTATGCTGTTTAATTGGA | 3865 | 93–131 | Saccharomycetales (Candida) |
| TCTGACGACAACAATGTAACGCCTG | ||||
| mtSSU rRNA | TTCGATACCCGTGTAGTTCTAGTAGTAAACTCATTATTGCTAACGTACTCTTCAGGTGG | 3867 | 113–127 | Saccharomycetales (Candida) |
| mtSSU rRNA | TACCAAGCCAATGACGAGTAGGG | 4145 | 87 | Tremellales (Cryptococcus) |
| TGCTGCACATAATCTATGCTCTGGAC | ||||
| hpr | TGACGAGTTCATGAGGGCAGGC | 4437 | 77 | Extraction control (pumpkin DNA) |
| TCTGGCCTTTCAGCAAGTTTCCAAC |
a
LSU, long subunit; SSU, short subunit; mt, mitochondrial.