Fig 5.

CITFA-7 is essential for RNA pol I transcription. Cotranscriptions of VSG ES promoter template VSG-trm and RNA pol II control template SLins19 in extracts that were mock depleted (mock), immunodepleted (depl) of CITFA-2 or CITFA-7, and reconstituted with buffer or final eluates (elu) of PTP–CITFA-2 or CITFA-7–PTP TAPs (top panel). Anti-CITFA-2 (middle panel) and anti-CITFA-7 (bottom panel) immunoblots of depleted and reconstituted extracts detecting endogenous, PTP-tagged, and P-tagged (after TEV protease digest) proteins are shown.