Fig 3.

Phenotypic characterization and the localization of GFP-AnPcpA in the alcA(p)::GFP-AnpcpA strain CPA02. (A) Outline of the strategy for creating the functional strain CPA02 by highly efficient homologous gene integration. (B) PCR analysis showing the integration of pLB01-AnpcpA5′ into the genome at the original AnpcpA locus in CPA02. (C) Colony phenotype comparison of CPA02 cultured on the solid inducing medium MMGTPR, the derepressing medium MMGPR, and the repressing medium YAG for 48 h at 37°C. (D) Germling phenotype comparison of CPA02 cultured in MMGPR and YAG for 9 h at 37°C. (E) Localization pattern of GFP-AnPcpA in CPA02 in different media. The nuclei were stained with DAPI. Arrows indicate GFP-AnPcpA, which was found occasionally on opposite ends of the elongated nucleus. Bars, 5 μm.