Fig 5.

AnPcpA is involved in the maintenance of septum shape but not required for actin ring formation and chitin disposition. (A) The mycelium phenotypes of wild-type WJA01 and CPA02 stained with DAPI and CFW. (Top) Conidial spores of WJA01 were cultured at 37°C for 10 h; (bottom) hyphal phenotypes in CPA02 cultured in the repressing medium YAG for 4 h after being cultured in the inducing medium MMGPR for 10 h. (B) Actin ring visualized by indirect immunofluorescence at sites of septum formation (arrow) in CPA02 after a shift from the inducing medium MMGPR to the repressing medium YAG for 4 h. Bars, 5 μm.