Fig 4.

Nematodes were infected with the C. albicans PES1/PES1 isogenic parental strain or with the C. albicans tetO-PES1/pes1 strain and transferred to liquid medium either with or without doxycycline (Doxy; 20 μg/ml). (A) The conditional tetO-PES1/pes1 strain was able to kill the worms at rates similar to those of the control strain, irrespective of the presence or absence of doxycycline. Error bars represent the standard deviations of the results determined with two replicate experiments. The data in panel A are from a single experiment representative of two independent biological replicates. (B) Microscopic observations indicated that killing of nematodes by cells of the C. albicans tetO-PES1/pes1 strain was associated with hyphal formation, in both the presence and the absence of doxycycline.