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. 2012 Dec;11(12):1451–1462. doi: 10.1128/EC.00268-12

Fig 5.

Fig 5

Recombinant CrLIP1 hydrolyzes phosphatidylethanolamine, the major membrane lipid of E. coli. (A) Thin-layer chromatographs of total lipids extracted from E. coli cells. Note the CrLIP1-dependent production of a significant increase of free fatty acids in the E. coli lysate. The arrow points to an unidentified lipid. PL indicates polar lipids. (B) TLC of PtdEtn hydrolysis reactions. The CrLIP1 protein causes the reduction of the PtdEtn substrate and produces free fatty acids and lyso-PtdEtn. The markers include PtdEtn and oleic acid from a commercial source and lyso-PtdEtn generated by the Rhizopus lipase (RL). (C) Quantitative analysis of CrLIP1-dependent PtdEtn hydrolysis in E. coli. Data are presented as the ratio of fatty acids in each lipid to fatty acids in the total lipid extract. Averages and SDs are shown by the ranges at the tops of the bars in the graph (n = 3).