Fig 1.

Snf1 and Por1 interact in the two-hybrid system. Strain CTY10-5d with an integrated lexAop-lacZ reporter was cotransformed with pairs of plasmids expressing the indicated proteins. β-Galactosidase activity was assayed in mid-log cultures grown in selective medium with high (2%) glucose (open bars) and shifted for 3 h to an otherwise identical medium with low (0.05%) glucose (closed bars). Values are averages for at least three transformants. Error bars indicate standard errors. Asterisks indicate statistically significant differences from the corresponding control values (P < 0.03 both in high and low glucose). (A) LexA-Por1 interacts with VP16-Snf1. (B) LexA-Snf1 interacts with GAD-Por1.