Fig 2.

G6PDH activities separated on native gels for wild-type and mutant strains. (A) G6PDH from the MW179-1D strain (wt) and its isogenic respiratory mutants. (B) G6PDH from glycolytic and respiratory mutants in the CBS2359 context. (C) G6PDH from CBS2359 grown on increasing concentrations of glucose and from the MW179-1D strain transformed with the RAG1 multicopy plasmid. Extracts were prepared from late-exponential-phase cultures grown on YP medium containing glucose (D), ethanol (E), or glycerol (G) at 2% or at the concentration of glucose indicated in the figure. Glucose at 0.5% plus 2% ethanol (DE) was added to cultures of Klsdh1Δ and Klcox14Δ mutants unable to grow on respiratory carbon sources. Cell extracts were fractioned on polyacrylamide gels and stained for G6PDH activity. The values within the boxes in panels B and C indicate the amount (as a percentage) of each G6PDH band in each lane, as determined by densitometric analysis using the Molecular Imaging software (Kodak).