Fig 3.

G6PDH activities separated on native gels for wild-type and mutant strains. (A) G6PDH from MW98-8C and its single isogenic adh mutants. (B) G6PDH from the adh° mutant, from the same strain with the KlADH1 or KlADH2 gene reintegrated into its chromosomal locus or from the adh° mutant transformed with a multicopy plasmid containing KlADH1. (C) G6PDH from the CBS2359 strain transformed with multicopy plasmids containing the genes indicated in each lane. Cell growth, cell extracts, gel conditions, and staining for G6PDH, as well as the values in the boxes, are as those described for Fig. 2.