Fig 5.

Sequence alignment of G6PDH from K. lactis and H. sapiens, performed using Clustal X. The two proteins share 46% sequence identity. The fully conserved residues are shown by asterisks. The conservation of residues involved in the interaction with the coenzyme NADP⁺ (inverted black triangle), the substrate glucose-6-phosphate (inverted white triangle), the structural NADP⁺ (open circles), and in tetramerization (black circles) is shown. The structural model of K. lactis G6PDH was automatically built using as a template either one of the two crystal structures available for the human enzyme (PDB IDs 2BH9 or 1QKI). Both structures are from proteins missing the first 11 (1QKI) or the first 26 (2BH9) residues in the sequence.