Table 1.
Yeast strains and DNA primers used in this study
| Strain or target genes and primers | Genotype or sequence | Reference or source |
|---|---|---|
| K. lactis strains | ||
| CBS2359a | MATa | CBS collection |
| GG1993 | CBS2359 Klpda1::Tn5BLE | 42 |
| GG1996 | CBS2359 Klpgi1::loxP (rag2Δ) | 36 |
| CBS2359/152Fa | MATa metA1 ura3 | 29 |
| CBS2359/152F/2 | CBS2359 Klpdc1::URA3 metA1 ura3 | 29 |
| MW98-8C | Matα lysA1 argA1 ura3 rag1 rag2 adh3 | 12 |
| CF1 | MW98-8C Kladh1::URA3 | 25 |
| CF2 | MW98-8C Kladh2::URA3 | 25 |
| MW179-1D | Matα metA1 ade-T600 leu2 trpA1 ura3 lac4 | 11 |
| MW179-1D/Klcox14Δ | MW179-1D Klcox14::KanMX4 | 11 |
| MW179-1D/Klnde1Δ | MW179-1D Klnde1::KanMX4 | 35 |
| MW179-1D/Klndi1Δ | MW179-1D Klndi1::KanMX4 | 35 |
| MW179-1D/Klsdh1Δ | MW179-1D Klsdh1::KanMX4 | 32 |
| MW179-1D/Klzwf1Δ | MW179-1D Klzwf1::KanMX4 | 33 |
| MS7-62b | Matα lysA1 argA1 Kladh1Δ Kladh2Δ Kladh3Δ Kladh4Δ | 30 |
| MS7-62/KlADH1 | Matα lysA1 argA1 Kladh2Δ Kladh3Δ Kladh4Δ | 15 |
| MS7-62/KlADH2 | Matα lysA1 argA1 Kladh1Δ Kladh3Δ Kladh4Δ | 15 |
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Euroscarf collection |
| Target gene and primer | ||
| KlALD4 | ||
| Forward primer | GGGAGCTCTTTCAACTACCAAGTATCGT | |
| Reverse primer | GGGAGCTCCACAGGATATTCAACGCCAT | |
| KlALD6 | ||
| Forward primer | GGGTCTAGAGTTGCTGGCTGTGGTGTC | |
| Reverse primer | GGGTCTAGATGTGATCACCGATTGTTCG | |
| KlGPD1 | ||
| Forward primer | GGGTCTAGAACTGCGTGGGATGGGCG | |
| Reverse primer | GGGTCTAGAGGAAACGAGAGTTGTTACCC | |
| KlZWF1GFP 5′ | ||
| Forward primer | AGGGTCGACACTGTATTCCTCTCGTTACC | |
| Reverse primer | GGGGATATCCATTTTAGGAGTGGTGA | |
| KlZWF1GFP 3′ | ||
| Forward primer | AGGGTCGACACTGTATTCCTCTCGTTACC | |
| Reverse primer | GGGGATATCACTGAAAGCTCTATCGT |
a
These strains had identical G6PDH patterns.
b
In the text, this strain is also referred to as adh°.