Fig 4.

Hsl1p kinase activity is required for degradation of septin-tethered Swe1p. (A) Schematic of Hsl1p. The K110R point mutation in the kinase domain is noted by an asterisk. (B) Septin-Swe1p is localized to the bud neck in hsl1^K110R cells, but GFP-Swe1p is not. Cells of strains DLY12973 and DLY15597 were grown at 30°C and photographed. (C) Swe1p and septin-Swe1p are both stabilized in a strain harboring the kinase-dead hsl1^K110R mutant. A single-cycle synchrony experiment, as described in the legend of Fig. 1C, was performed with hsl1^K110R strain DLY12973. (D) Synchrony parameters (budding and nuclear division) for the experiment depicted in panel C were scored for >200 cells per sample.