Fig 1.

Retention of glycerol by C. glabrata in response to hyperosmotic stress and in fpsΔ mutants. Glycerol concentrations of log-phase cells were determined in triplicate. C. glabrata strains were grown to mid-log phase in YPD and diluted into YPD with or without sorbitol (to 1.8 M) to induce hyperosmotic shock (15 min). Each value represents the mean and standard deviation from three independent experiments. Strains were the wild type (BG14) and fps1Δ (DL3616), fps2Δ (DL3675), and fps1Δ fps2Δ (fps1Δ2Δ) (DL3679) mutants. Statistical significance was determined using two-sample t tests with a two-tailed distribution. ODU, optical density units; **, P < 0.005.