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. 2012 Dec;11(12):1512–1519. doi: 10.1128/EC.00231-12

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Fig 5 — Heterologous complementation of S. cerevisiae fps1Δ and rgc1Δ rgc2Δ (rgc1Δ2Δ) mutant phenotypes by C. glabrata genes. (A) Complementation of an fps1Δ mutant. S. cerevisiae FPS1 was replaced by integration with the indicated FPS genes such that the integrated genes were expressed under the control of the endogenous ScFPS1 promoter. The indicated strains were streaked onto YPD plates with or without arsenite (As) and incubated at 40°C or 30°C, respectively, for 3 days. Strains were the wild type (DL3187) and fps1Δ (DL3226), fps1::ScFPS1 (DL3799), fps1::CgFPS1 (DL3800), and fps1::CgFPS2 (DL3801) mutants. (B) Complementation of an rgc1Δ rgc2Δ mutant. The RGC2 gene of S. cerevisiae was replaced by integration with the indicated RGC genes in an rgc1Δ mutant background such that the integrated genes were expressed under the control of the endogenous ScRGC2 promoter. Temperature and arsenite phenotypes were determined as described for panel A. Strains were the wild type (DL3187) and rgc1Δ rgc2Δ (DL3207), rgc1Δ rgc2::ScRGC2 (DL3802), rgc1Δ rgc2::CgRGC1 (DL3803), and rgc1Δ rgc2::CgRGC2 (DL3804) mutants.