Fig 10.

α₁-ARs induce hypertrophic gene transcription through the AKAP-Lbc-IKKβ complex and IL-6. (A) Real-time PCR analysis of ANF and β-MHC expression was performed on total RNA preparations obtained from NVMs that were serum starved for 48 h and subsequently incubated for 24 h in the absence or presence of 10⁻⁴ M PE and IL-6 neutralizing antibodies (IL-6 Ab). Results are expressed as means ± SE from eight independent experiments. *, P < 0.05. (B) Single-cell real-time PCR analysis of ANF, β-MHC, and IL-6 expression was performed on total RNA preparations obtained from FACS-sorted NVMs that were infected as indicated in the legend to Fig. 5A and subsequently transfected with rescue constructs encoding mCherry-tagged AKAP-Lbc or AKAP-Lbc W2328L. Results are expressed as means ± SE from six independent experiments. *, P < 0.05.