Fig 7.
AKAP-Lbc-IKKβ complex mediates α1-AR-induced cardiomyocyte hypertrophy and sarcomere reorganization. Rat NVMs were infected with lentiviruses carrying GFP (control) or both GFP and AKAP-Lbc shRNAs and subsequently transfected with the empty Flag vector or the cDNAs encoding human AKAP-Lbc and AKAP-Lbc W2328L. Forty-eight hours after transfection, cells were incubated for 24 h with 10−4 M PE. Cells were then fixed, permeabilized, and incubated with mouse anti-α-actinin (A) or anti-ZASP (B) antibodies followed by rhodamine-conjugated donkey anti-mouse secondary antibodies to visualize the sarcomeres. The expression of Flag-AKAP-Lbc was detected using rabbit anti-Flag polyclonal antibodies and AMCA-conjugated donkey anti-rabbit secondary antibodies, whereas GFP expression was visualized directly by fluorescent excitation at 490 nm.